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mdabrarfaiyaj/README.md

Hi, I'm Md Abrar Faiyaj 👋

Junior Research Collaborator | Computational Biologist | Bioinformatics Analyst
Building reproducible pipelines and interactive dashboards for infectious disease genomics and AMR surveillance

ORCID Email LinkedIn


🔬 What I Work On

flowchart LR
    A[Biological Question\nAMR · Genomics · Epidemiology] --> B[Data Collection\nGEO · SRA · TyphiNET · NCBI]
    B --> C[Reproducible Pipeline\nR · Bash · DESeq2 · HISAT2]
    C --> D[Statistical Analysis\nDEG · AMR Trend · Variant Surveillance]
    D --> E[Interactive Visualization\nShiny Dashboard · Quarto Report]
    E --> F[Communication\nGitHub Pages · LinkedIn · Documentation]
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I work at the intersection of infectious disease biology and computational data analysis — turning messy genomic and epidemiological datasets into reproducible, interactive tools that researchers can actually use.


🧰 Technical Toolkit

Languages & Frameworks

R Python Bash SQL

Bioinformatics & Genomics

DESeq2 HISAT2 featureCounts SPAdes Galaxy

Visualization & Dashboards

Shiny Quarto ggplot2 Plotly

Tools & Reproducibility

Git GitHub renv Linux Posit Cloud


🚀 Featured Projects

1. Typhoid Fever AMR Surveillance Dashboard — Bangladesh (1999–2022)

A 24-year retrospective analysis of Salmonella Typhi antimicrobial resistance trends in Bangladesh, extending Tanmoy et al. 2024 (PLOS NTDs) with TyphiNET genomic data. Fully deployed as an interactive Shiny dashboard and a Quarto report on GitHub Pages.

Highlights:

  • Integrated clinical AMR phenotype data with whole-genome sequencing genotype data from TyphiNET
  • Visualized multi-drug resistance (MDR), XDR, and H58 lineage trends over 24 years
  • Deployed interactive Shiny dashboard with tabbed navigation and downloadable outputs
  • Published reproducible Quarto report with embedded figures and methodology

Tech: R Shiny ggplot2 Quarto renv GitHub Pages TyphiNET AMR genomics

Typhoid AMR Dashboard

🔗 View Repository · 📊 Live Dashboard · 📄 Quarto Report


2. RNA-Seq Replication — Shemer et al., Immunity 2020 (GSE157234)

Reproduced bulk RNA-Seq analysis from a high-impact immunology paper on IL-10 receptor-deficient microglia. Discovered that the GEO deposit contains UTAP-normalized counts (not raw counts) — a methodological finding documented publicly and shared with the research community, attracting engagement from scientists at Illumina, Novartis, Pfizer, and GSK.

Highlights:

  • Replicated differential expression analysis using DESeq2 with apeglm shrinkage
  • Identified 1,563 DEGs with clean mutant/control separation
  • Documented GEO data transparency issue publicly, reaching 6,300+ LinkedIn impressions
  • V2 pipeline in progress from raw FASTQs via SRA for full methodological rigor

Tech: R DESeq2 apeglm ggplot2 pheatmap Shiny GEO SRA Bioconductor

RNA-seq Replication Results

🔗 View Repository · 📊 Live Dashboard


3. Bangladesh Dengue Genomic Surveillance Dashboard

Interactive R Shiny dashboard analyzing DENV-2 sequences from the 2023 Bangladesh dengue outbreak (Dhaka & Chattogram isolates). Includes QC filtering, motif detection, and comparative visualization.

Highlights:

  • Analyzed 13 real DENV-2 sequences from the 2023 Bangladesh outbreak
  • Detected conserved motifs and visualized variant patterns across isolates
  • Deployed interactive dashboard with modern tabbed UI

Tech: R Shiny Bioconductor sequence analysis genomic surveillance

Dengue Surveillance Dashboard

🔗 View Repository · 📊 Live Dashboard


4. Heart Disease Patient Data Analysis

Exploratory analysis of 1,025 clinical patient records to identify risk factors for heart disease. Combined Python + SQL workflow with a six-panel diagnostic visualization dashboard.

Highlights:

  • 8 standalone SQL queries on SQLite for structured data retrieval
  • Identified maximum heart rate as the strongest predictor; challenged cholesterol assumptions
  • Fully documented, reproducible Jupyter Notebook with biological interpretation

Tech: Python SQL SQLite Pandas Matplotlib Seaborn Jupyter

Patient Data Visualization

🔗 View Repository


📚 Research Interests

  • Infectious Disease Genomics & AMR Surveillance
  • RNA-Seq & Multi-omics Data Analysis
  • Reproducible Bioinformatics Tools & Workflows
  • Public Health Data Science
  • Computational Biology & Interactive Dashboards

📫 Let's Connect

Open to research collaborations, bioinformatics roles, and PhD opportunities in computational biology and infectious disease genomics.


Last updated: June 2026

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  1. Typhoid-Fever-in-Bangladesh Typhoid-Fever-in-Bangladesh Public

    Reproducible re-analysis of 24-year Salmonella Typhi AMR surveillance in Bangladesh (Tanmoy et al. 2024, PLOS NTDs). Phenotypic trends from 12,435 isolates + genomic validation via TyphiNET. Intera…

    HTML

  2. rna-seq-shiny-pipeline rna-seq-shiny-pipeline Public

    RNA-Seq Differential Expression Analysis with DESeq2 + Interactive Shiny Dashboard

    R 2 1

  3. bangladeshi-dengue-strain-tracker bangladeshi-dengue-strain-tracker Public

    Interactive R Shiny dashboard analyzing 13 real DENV-2 sequences from the 2023 Bangladesh dengue outbreak (Dhaka & Chattogram isolates). Includes QC filtering, motif detection, modern visualization…

    R 1

  4. patient-data-analysis patient-data-analysis Public

    Exploratory data analysis of 1,025 clinical patient records to identify risk factors associated with heart disease using Python, SQL, and data visualization.

    Jupyter Notebook

  5. dengue-variant-tracker-prototype dengue-variant-tracker-prototype Public

    Prototype version: General dengue motif & variant tracker using reference strains (NC_001477.1 Dengue Virus 1, complete genome, NC_001474.2, Dengue Virus 2,complete genome)

    R 1

  6. 01-penguins-biological-data-analysis 01-penguins-biological-data-analysis Public

    Morphometric analysis of Palmer Penguins demonstrating data cleaning, statistical testing, and visualization in R

    R