Metrics were mostly inspired by https://crisprdb.org/ go check them and their paper out. Wong N, Liu W, Wang X. WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol. 2015 Nov 2;16:218. doi: 10.1186/s13059-015-0784-0. PMID: 26521937; PMCID: PMC4629399. I gathered gRNA sequences from them and also from CHOPCHOP for the Homo Sapiens CDKN2A gene. Go check them out as well. Kornel Labun, Tessa G Montague, Maximilian Krause, Yamila N Torres Cleuren, Håkon Tjeldnes, Eivind Valen, CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing, Nucleic Acids Research, Volume 47, Issue W1, 02 July 2019, Pages W171–W174, https://doi.org/10.1093/nar/gkz365 Future Work: Considering the context of the gRNAs, their surroundings, and potential mismatches across the genome.