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DESCRIPTION

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github::GraceYoon/SPRING,
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github::waldronlab/curatedMetagenomicData
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URL: https://microbiome.github.io/OMATutorials/
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BugReports: https://github.com/microbiome/OMATutorials/issues/
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BugReports: https://github.com/microbiome/omatutorials/issues/
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VignetteBuilder: knitr
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DockerImage: ghcr.io/microbiome/OMATutorials:latest

_format.yml

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grid:
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sidebar-width: 400px
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body-width: 1000px
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bibliography: bibliography.bib
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bibliography: inst/bibliography.bib
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citation-location: margin

vignettes/gbcc2025.Rmd

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output:
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html_document:
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code_folding: hide
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bibliography: ../inst/bibliography.bib
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---
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```{r}
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This training session introduces Bioconductor tools for microbiome data science
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through a practical case study. It focuses on a framework built around the
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TreeSummarizedExperiment data container, designed for improved efficiency,
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`r BiocStyle::Biocpkg("TreeSummarizedExperiment")` data container, designed for improved efficiency,
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scalability, and data integration. Participants will gain hands-on experience
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with common analysis and visualization methods using the mia package family,
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along with other interoperable tools from the Bioconductor ecosystem. After the
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### _R_ / _Bioconductor_ packages used
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In this training session, we will cover a common methods and packages for
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microbiome data science in _SummarizedExperiment_ ecosystem. We will have
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specific focus on
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[the _mia_ package](https://bioconductor.org/packages/devel/bioc/html/mia.html),
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microbiome data science in `r BiocStyle::Biocpkg("SummarizedExperiment")` ecosystem. We will have
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specific focus on `r BiocStyle::Biocpkg("mia")`,
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which provides essential methods for conducting microbiome analysis.
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### Time outline
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#### Questions
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- What is _mia_ and _OMA_?
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- How microbiome data science is conducted in _SummarizezExperiment_ ecosystem?
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- How microbiome data science is conducted in `r BiocStyle::Biocpkg("SummarizedExperiment")` ecosystem?
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- What benefits this new ecosystem have compared to previous approaches?
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#### Objectives
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- **Analyze and apply methods**: Apply the _SummarizedExperiment_ ecosystem to process and analyze microbiome data.
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- **Analyze and apply methods**: Apply the `r BiocStyle::Biocpkg("SummarizedExperiment")` ecosystem to process and analyze microbiome data.
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- **Create visualizations**: Generate and interpret visualizations.
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- [iSEEtree](https://bioconductor.org/packages/release/bioc/html/iSEEtree.html) (Interactive visualization)
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- [Expanded by independent developers](https://microbiome.github.io/OMA/docs/devel/pages/miaverse.html#sec-packages)
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**Interoperable with the _SummarizedExperiment_ ecosystem**
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**Interoperable with the `r BiocStyle::Biocpkg("SummarizedExperiment")` ecosystem**
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:::
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)
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```
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Once loaded, we can see that the data includes several _TreeSE_ objects, which
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Once loaded, we can see that the data includes several `r BiocStyle::Biocpkg("TreeSummarizedExperiment")` objects, which
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include taxonomy annotations and functional predictions. In this demonstration,
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we are only interested on the taxonomy annotations, so we select it from the
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list and store it into a variable named `tse`.
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#### Data container
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_TreeSE_ extends _SE_ class by adding a support for microbiome-specific
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`r BiocStyle::Biocpkg("TreeSummarizedExperiment")` extends `r BiocStyle::Biocpkg("SummarizedExperiment")` class by adding a support for microbiome-specific
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datatypes. These include, for instance, `rowTree` slot that can be utilized to
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store phylogeny or any other hierarchical presentation of the data. All slots
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derived from _SE_ class are also available in _TreeSE_, providing full backward
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derived from `r BiocStyle::Biocpkg("SummarizedExperiment")` class are also available in `r BiocStyle::Biocpkg("TreeSummarizedExperiment")`, providing full backward
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compatibility.
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![](figures/TreeSE.png){width=80%}
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#### Community summaries
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While `mia` package include common methods for analysis, `miaViz` provides
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While `r BiocStyle::Biocpkg("mia")` package include common methods for analysis, `r BiocStyle::Biocpkg("miaViz")` provides
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methods for visualizing microbiome data. For instance, we can visualize
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abundance of phyla with a bar plot. To compare controls and CRC patients, we can
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visualize the groups separately.
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## Questions, discussion and recap
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1. Microbiome data science in _SummarizedExperiment_ ecosystem
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1. Microbiome data science in `r BiocStyle::Biocpkg("SummarizedExperiment")` ecosystem
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2. Scalable and computationally efficient
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3. Integration of multi-table and multi-omics datasets
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